![]() Used in first-strand cDNA synthesis for subsequent NGS analysis. The reduced g-anisotropy relative to the CoCo enzyme and clearly axial symmetry of the CoCd EPR are consistent with the more centrosymmetric environment of the pseudotetrahedral Zn 1 site. Used in first-strand cDNA synthesis for downstream NGS applications.Īn optimised reverse transcriptase for production of full-length cDNA from as little as 100pg of total RNA/low abundance RNA. MMLV High Performance Reverse TranscriptaseĪn RNA-dependent MMLV DNA polymerase which is highly efficient at producing full-length cDNA from RNA templates. Used in the preparation of cDNA libraries for small-RNA transcriptome analysis (eg. Ligates single-stranded adenylated DNA or RNA oligos to RNA targets for a variety of applications. Useful for screening introns in cDNA libraries. Useful in removing rRNA from total RNA samples prior to library construction.Ī 3′ to 5′ exoribonuclease that digests linear RNAs but spares lariat or circular RNA. Useful in MIP-Seq and similar applications.ĭegrades RNA in a DNA:RNA hybrid without affecting DNA or any unhybridised RNA at high reaction temperatures. Highly thermostable DNA ligase for applications where ligation at high temperatures is beneficial. Used in combination with UNG (UDG) to generate a single nucleotide gap (with terminal 5' and 3' phosphates) at a uracil site. Used in combination with Endonuclease VIII to generate a single nucleotide gap (with terminal 5' and 3' phosphates) at a uracil site.Īcts as both an N-glycosylase and an AP-lyase in releasing damaged pyrimidines in DNA. The resulting abasic sites are susceptible to cleavage. ![]() Used in the circularisation of ssDNA that can be used as templates for rolling-circle replication.Īlso known as UDG, UNG catalyses the release of uracil from single and double stranded uracyl-containing DNA. Thermostable ligase that catalyses the intramolecular ligation of ssDNA templates. Used in second-strand cDNA synthesis and strand-displacement amplification. Adds an ʻAʼ base to the 3' end of the blunt phosphorylated DNA fragments, using the polymerase activity. Used in DNA sequencing to label the 5′ end of DNA and RNA with 32P or 33P.Įxo-Minus Klenow DNA Polymerase (D355A, E357A)Ī Klenow DNA polymerase that lacks all 5′ to 3′ and 3′ to 5′ exonuclease activity. Phosphorylates 5′ ends of ssDNA, dsDNA, RNA and nucleoside 3′ monophosphates. ![]() Used to form blunt ends by removing 3′ overhangs or filling in 5′ overhangs. Workhorse enzyme for adapter ligation to build complex libraries.Īn E.coli DNA polymerase I fragment that lacks 5′ to 3′ exonuclease activity but retains 3′ to 5′ exonuclease activity and 5′ to 3′ polymerase activity. T4 DNA Ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. ![]() Start with a small batch for your pilot studies and scale up to bulk orders when you’re ready. ![]()
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