![]() Miami (MIA) – Miami International Airport.Los Angeles (LAX) – Los Angeles International Airport.Las Vegas (LAS) – Las Vegas Harry Ried International Airport.Kansas City (MCI) – Kansas City International Airport.Houston (IAH) – George Bush Intercontinental Airport.Halifax (YHZ) – Halifax Stanfield International Airport.Fort Lauderdale (FLL) – Fort Lauderdale-Hollywood International Airport.Edmonton (YEG) – Edmonton International Airport.Denver (DEN) – Denver International Airport.Dallas (DFW) – Dallas Fort Worth International Airport.Chicago (ORD) – Chicago O’Hare International Airport.Calgary (YYC) – Calgary International Airport.Boston (BOS) – Boston Logan International Airport.Baltimore (BWI) – Baltimore-Washington International Airport.Atlanta (ATL) – Hartsfield-Jackson Atlanta International Airport.The current airport locations where you can use Mobile Passport Control are below: airports, 3 Canadian preclearance locations, and 4 cruise ports around the country. Mobile Passport Control is currently available at 31 U.S. Which Airports Use Mobile Passport Control? However, when you click it, you’ll be directed to download the new (as of February 2022) CBP MPC app. Hot Tip: The original Mobile Passport app, developed by Airside, still shows on the Apple and Android app store. You can even set up a profile for each family member within a single account, which is especially nice for families with young children. In addition, you can securely store your passport and personal information on the app and use the same information on multiple trips. The information sent from the app replaces the paper forms passengers typically have to fill out before arrival. All you have to do is download and use the Customs and Border Protection Mobile Passport Control (CBP MPC) app (available for iOS and Android). You don’t need to have a pen or fill out arrival forms on the plane or in line. Customs and Border Protection checkpoints when returning to the U.S. The program is designed to help travelers move more quickly through U.S. Mobile Passport Control (MPC) is a program authorized by U.S. How To Use the Mobile Passport Control App.Which Airports Use Mobile Passport Control?.IHG Rewards Club Loyalty Program Review.Choice Privileges Loyalty Program Review.Marriott Bonvoy Hotel Loyalty Program Review.How to Buy Hotel Points & Airline Miles. ![]() Best Ways to Redeem Choice Hotel Points.Best IHG Luxury Hotels to Book With Points.Best Ways to Redeem Marriott Points for Hotels.Best Marriott Luxury Hotels to Book With Points.Guide to Hotel Status Matches and Challenges.Best Hilton Luxury Hotels to Book With Points.Best Websites for Booking Affordable Hotels.TSA PreCheck – Everything You Need to Know.How to Cancel an American Airlines Flight.Best Crossbody Bags & Purses for Travel. ![]()
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![]() ![]() Roman Polanski was nominated for awards by the Writers Guild of America and the Directors Guild of America. It also won Best Supporting Actress at the Golden Globes and received two other nominations. The film earned several accolades that are incredibly rare for the genre - it won Best Supporting Actress and was nominated for a Best Screenplay at the Academy Awards. Rosemary’s inescapable situation and paranoia are palpable. Mia Farrow gives a convincing performance as Rosemary, a soon-to-be mother who discovers a satanic coven is after her baby. Rosemary’s Baby (1968) A satanic coven seeks to acquire Rosemary’s newborn baby.Ī movie so iconic that it was selected for preservation in the National Film Registry by the Library of Congress. (“Polygynous” means that a man can have multiple wives as well as sex partners.) The family quickly senses danger and tries to extricate themselves from the hands of…the hands of the man who holds their fate. ![]() In short, a family gets lost driving around in the desert and encounters a polygynous pagan cult led by someone known as The Master. Even its name is dumb-“manos” means “hands” in Spanish, so the film’s title literally translates as Hands: The Hands of Fate. Manos: The Hands of Fate (1966) This bizarre movie features a polygynous pagan cult in Texas.Ī notoriously bad film-so bad that even its director called it the worst film ever made-this was featured on Mystery Science Theater 3000, whose creators also called it the worst movie they’d ever had to mock. I give thee my soul, take me into thy service. For all eternity shall I sacrifice unto thee. For all eternity shall I practice the ritual of Black Mass. I have made my pact with thee O Lucifer! Hear me, hear me! I will do thy bidding for all eternity. The following incantation by one of the followers caused the film to be banned in several places: Upon her arrival, she realizes that witchcraft is anything but dead in the town. The City of the Dead (1960) An old cult movie from the 1960s.Ī young female college student with an interest in the occult pays a visit to the fictional town of Whitewood, Massachusetts, where a witch had been burned at the stake in 1692. ![]() As he plans to sacrifice a beautiful blonde woman to Satan during the New Moon, Lugosi’s character attempts to save the day. Karloff plays the leader of a satanic group who takes his orders from a book called The Rites of Lucifer. ![]() Horror legends Bela Lugosi and Boris Karloff team up in what is thought to be the first horror film to depict satanism as a cult. Movies about Cults The Black Cat (1934) The Black Cat is widely considered the first time cults were portrayed in cinema. So what’s scarier: another person controlling your fate, or a group with no physical leader to take down? Here are 50+ horror movies to help you find out. Movies have explored every aspect of these strange groups - the manipulation and brainwashing by human beings, occultism and the Devil, political and doomsday groups - you name it. Like cults, mass hysteria isn’t new and it’s easy to see how one small outcry can turn into something unstoppable - particularly when it involves an otherness. The Satanic Panic of the 1980s still permeates our society even though all claims of ritualistic abuse remain unfounded, equivalent to a modern-day witch hunt. There’s also a fear beyond this classification. Would we recognize it? Would we be willing to make a commotion? That’s what makes this kind of cult so terrifying - it could happen in our very own organizations. By the time a follower realizes that something sinister is underway, it’s too late. They start out just like any other church or collective by reeling their members in with the promise of unconditional love and community. So how does this keep happening? It’s simple, really. We’ve witnessed many groups that fit this bill in recent history: the Manson Family, the Branch Davidians, Jonestown, Heaven’s Gate, NXIVM, etc. Economic, sexual, and other exploitation of membersĪ satanic ritual happens in To the Devil a Daughter, a horror fan favorite from the 70s.In a 1981 edition of the Harvard Mental Health Letter, psychiatrist Robert Jay Lifton defined three characteristics of a cult: The term cult comes from the Latin word cultus, which means to worship a deity, but in the last few centuries its colloquial definition has transformed. The fear (and fascination) with cults is nothing new and movies about cults have been around since we started making films. ![]() ![]() ![]() If you find any problems with the app, please post them below. The app shouldn't cause any problems, but it is an "use at your own risk" app. It does actually work with the latest version of Android, so I'm releasing it here for free and embarking on a total rewrite for AM23. I can't do that without updating to the latest version of the programming language I'm using, and I can't update without a complete rewrite of the app. This version was supposed to be going onto Google Play, but it needs to comply with their newer (more restrictive) file permission model to be accepted. Here's an overview of the app (from last year, but pretty much the same): After that, the app should remember the folder and be able to show the buttons.Īt this stage I'm not sure I can fix in AM22, the open file logic is pretty complicated and needs a total rewrite (which it will get for AM23 but using a solution that's not compatible with AM22). It should then show the save file buttons.Īfter that, everything should work fine. This is only a problem the first time you open the app and choose the folder. You need to close the app fully (back button and say Yes) and then open it again. ![]() It SHOULD then take you back to the main screen and show you buttons for each save file BUT there is a bug and it doesn't show them. Note it won't show any files, only folders. You then need to find the Games folder and press the green tick / check mark in the top right corner. You will be prompted with the Find Folder button. If it doesn't find any save files, you will need to tell the app where the save file folder is and there is a serious bug here. If it finds them, you will see buttons for each save file found. Once opened for the first time, the app will look for save files in a couple of common places. You will need to allow APKs to be installed from sources other than Google Play and you will also be prompted to allow installation from this source. This will be released on Google Play and will not be free. I plan to release a new app called AM23 (for FMM23). NOTE: This will only work with FMM22, it will NOT work with FMM23 when it comes Search for players by many parameters (CA, PA, Age, position, foot, name, club, some hidden attributes etc).Įxport the current view of players to a FMM shortlist so you can import them into your FMM shortlist See the CA, PA, hidden attributes etc for any player This is a scouting app for FMM22 (Android), letting you: Always back up your files and I take no responsibility for what happens if you don't! When you want to go back to the original game, just delete all the new files and empty the cache again, then Reload Skin and it should be fine. I'm happy to help anyone who has problems but it's really important to follow the steps above correctly. Start a new game with Denmark and whatever other leagues you want. Go to Settings and then User Interface, and press Reload Skin at the bottom. Go to android/data//files/installed/application_support/Sports Interactive/Football Manager 2022 Mobile/normal/caches and delete the file in there - there should only be one file and it will have a name like fmhi_hdtv_skin.skc ![]() Paste the "changes.txt" in android/data//files/installed zip folder into the new folder you created "database". zip folder and the changes.txt.Įxtract all the files from the. If that folder already exists, empty it by deleting the files in it.ĭowload the files in this post - the. Go to android/data//files/installed and create a new folder called "database". Set the news filter to National, and I recommend deleting the Danish league logos for realism but that's up to you.Īndroid only - I don't know how to do this for iPhone but am willing to help anyone who wants to try.īackup your game - this will take over your version of FMM so you might not be able to continue saved games. So follow the instructions then when you are starting a new game, load Denmark and whatever backup Leagues you want. Instructions are the same as last time, except we're using the Danish League system this time. Let me know if you spot any missing or mistaken ones. ![]() It's back again, the Global Hyper Mega League! And given the game is unlikely to get any more updates, it should be stable forever. ![]() ![]() (2) The second need spelled out in this first request is that the message may continue to be glorified. If the message is to run swiftly, it must have the sovereign work of God make clear the way (cf. Second, the very nature of such a request calls to mind the fact that Satan and this world is always at work to throw up hindrances or barriers against the message. Again, we get a glimpse of Paul’s confidence. Note first that the focus here is on the message rather than the messenger for in the final analysis it is the message that transforms people. This is the present continuous tense of the Greek word trecho, which means literally, “run” but it is used figuratively of “proceeding quickly without hindrance.” This is, then, a prayer that God’s message will continue to progress swiftly and without hindrance to and within the hearts of men and women as it had done so powerfully among the Thessalonians (see 1 Thes. The First Request: Literally, “that the message ( ho logos, the word) of the Lord may run and be glorified just as also with you.” The message of the word is the message of gospel, the divine revelation which God breathed and which thereby comes with God’s authority behind it. Significantly, both of these requests ultimately related to the progress of the gospel, even though the second request was more personal. They did not just ask for prayer in general (i.e., pray for us), but they were specific with two primary requests seen in the clauses introduced by “that” (two hina clauses introducing the content of the request). The content of their prayer is spelled out in two specific requests. Thus, recognizing their own inadequacy and need of God’s enablement, the apostle and his team humbly sought the prayer support of others. Paul gave us more books of the New Testament than any other of the apostles or their associates, yet this great man of God humbly sought the prayer of those he and his team had led to Christ and were teaching to help them grow in Christ. Here was a giant among men and one used mightily by the Lord. There is an important lesson in this for all of us. Too much Christian work is attempted today by human plans and promotion with far too much dependence on the methods employed and in the dynamic personalities of people rather than on a prayerful dependence on the Lord. Thus, it was not unusual for Paul, who consistently prayed for his converts, to ask for prayer himself (see Rom. 5:25) puts some emphasis on the request and the sense of Paul’s awareness of his need of God’s hand and the important part the prayer of the saints has on our ministries. ![]() He says, “Pray, brethren (the plural adelphoi used as a vocative of address), for us.” The use of the vocative (as in 1 Thes. 88Īs is 1 Thessalonians 5:25, Paul asks for prayer on behalf of their missionary team. With the word “finally” (Greek loipon, a particle of transition often found toward the end of a letter) the apostle indicates that he is coming to the close of the letter, but not necessarily implying that he was immediately ending the letter or that other matters might not be discussed. ![]() Paul’s Confidence in Prayerģ:1 Finally pray for us, brothers and sisters, that the Lord’s message may spread quickly and be honored as in fact it was among you, 3:2 and that we may be delivered from perverse and evil people. Again, the apostle provides us with a model, not just for ministry but for life. This is not only the position of wisdom but of humility as we put our trust not in ourselves, but in a sovereign God and Savior. Our modern ‘go-go’ tendency is to be quick to plan and act rather than pray, wait on the Lord, and then in God’s timing and leading, work in His strength, leading, and provision. The Lord Jesus said emphatically, “I will build my church and the gates of Hades will not prevail against it.” While God uses frail human instruments in accomplishing His work on earth, the ultimate accomplishment of the work depends on the work and faithfulness of the Lord and His Word. Their confidence for whatever they might need and face was an unending trust in the provision and faithfulness of the Lord and His powerful Word. With chapter 3 Paul begins to bring this epistle to a close, but in doing so, we are privileged to see a wonderful model as Paul demonstrates how his team’s confidence lay not in human plans, promotion, programs, or human personalities, but in the Lord Himself. ![]() ![]() ![]() Intro Offer: Unlimited Cashback Match - only from Discover. ![]() Click through to Discover's website to see terms and conditions.Get an alert if we find your Social Security number on any of thousands of Dark Web sites.Freeze your account in seconds with an on/off switch either on the mobile app or website to prevent new purchases, cash advances, and balance transfers.Discover is accepted nationwide by 99% of the places that take credit cards.Discover could help you reduce exposure of your personal information online by helping you remove it from select people-search sites that could sell your data.Then 16.74% to 27.74% Standard Variable Purchase APR will apply. 0% intro APR for 15 months on purchases.However you redeem, Miles keep the same value. 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If you get antsy waiting on the status of a Discover card application, there are ways you can check on your application. That can be done by calling the Discover customer service phone number as well. However, you will have to indicate to Discover why you feel you should have been approved. You can also ask that Discover reconsider your application if you’ve been denied. If you find out that your application has been denied, you will be able to find out the reason. Once you do that, you can listen to your application status. Then, you will be asked to enter your Social Security number. You should push # on the first menu list provided, and four on the second list, although make sure you listen to the prompts because those options can change. The line is available at all times, and a real person should take your call.įor an automated version, there are menu options when you call the number. If you prefer to check your status by phone, you can call Discover’s customer service phone number at (800) 347-2683. That will immediately provide your Discover card application status. The best way to check your application status is to go to the card application site, where you enter your social security number and zip code. The following are some simple steps you can take to check the status of your Discover card application. How to Check a Discover Card Application Status So, in the meantime, you can check the status of your credit card application. You may want to plan for whether you will have access to that credit or not. Sometimes you may not want to wait to receive that information in the mail. If you’re denied, you’ll receive a letter in the mail from Discover letting you know why your application was denied. If you are approved, even if you don’t hear back immediately, you’ll receive your new Discover card in the mail. They will look at your payment history, your debt, and whether you’ve recently applied for new credit with any other companies. Once that occurs, and your identity has been verified, the issuing company will look at your credit history. If you don’t, you may receive a response letting you know that your application will undergo further review. Whether you’re applying for a Discover card or another type of card, the issuer will usually give you a response immediately, but not necessarily a final decision. After that, the process has several more steps before the lender accepts or declines you. Usually, your personal information and perhaps some information about your income and expenses are included. You can apply for a credit card by filling out an application online. You can take several steps to check your Discover credit card application status, which may end up in approval. However, if you don’t receive an automatic approval, that doesn’t mean you’re denied. When you apply for a Discover credit card, you may receive an automatic approval, but not always. ![]() ![]() ![]() Best of all, the manual legwork and redundant administration has been eliminated, making service fees highly competitive. Once approved, funds are typically deposited to your account within a matter of days. It takes just minutes to set up an account, and there’s no minimum or maximum funding requirements. Until you established a relationship with a factoring provider, you needed to have invoices verified for each new round of funding.įundThrough has revolutionized invoice factoring by integrating directly with a wide range of invoicing software.Traditional factoring companies also included no shortage of hidden fees. Traditional factoring companies typically purchased the invoices and handled collections, so clients and customers knew you had factored an invoice.Like payday loans, the fees were so cost-prohibitive that it was really only viable for occasional use as a stop-gap for cash flow emergencies.Historically, factoring had a few negatives: Giving you access to cash when it’s needed, without having to jump through the hoops of traditional lenders, or take on new debt. Generating investment capital so you can buy new equipment, build inventory, take on new and larger contracts, and otherwise grow your business on your terms. Improving your company’s cash flow so you can make payroll, pay your suppliers, and meet other financial obligations.Įmpowering you to take control of your funding and proactively plan to avoid any gaps or crises. Report a bug, suggest a feature request, or ask a question here. Use our help resources to learn which solution works best for your business. Our team is available Monday – Friday, 9am – 5pm ET. We’re always here to discuss how we can work together to help small businesses grow. We’re always looking for bright, passionate people to join our team.įundThrough in the press, and updates about our company. ![]() Get to know our story and why we work to help small businesses reach their full potential. Join us in our goal to help small businesses boost their working capital and grow. Read our thought leadership, case studies, company updates, and more. New to invoice funding? Read our thought leadership to help you boost your working capital. Read the success stories of small businesses that use invoice funding to expedite their growth. The factoring company (financier) purchases these invoices for a certain percentage of their total value and takes over the responsibility to collect payments. Get flexible invoice funding in days for one price without hidden fees.įundThrough has the highest customer satisfaction rate in the industry.ĭiscover the variety of B2B industries that get invoices paid in days to access capital Invoice Factoring: Also known as debt factoring, this particular type of financing enables businesses to avail capital by selling their unpaid invoices to a third-party factoring company. How FundThrough delivers streamlined invoice funding. Discover how easy it is to get invoices paid in days. ![]() ![]() ![]() The downside is that it is perhaps too slavish in adhering to Myth’s formula. There’s a unique tension and thrill to these hyper-realistic virtual battles against overwhelming odds. I keep saying it, but there just aren’t any other games that feel quite like this, and that’s a crying shame. It’s good because it’s a good formula that deserves to be repeated. The best and the worst thing about Nordic Warriors is that it so closely replicates the experience of Myth. On the one hand it takes some of the character out of the experience, but on the other hand I did lose a lot less units to friendly fire, which I can’t complain too hard about. NW seems a bit less stringent about realism than its predecessors - I didn’t notice shrapnel doing any damage, and arrows seem less likely to be blown off course - which is a mixed bag. Myth and Nordic Warriors also share a strong degree of realism, with projectiles following real physics. I had my shaman heal her up, and from that point on I did everything I could to keep Iona alive in all subsequent missions. ![]() She was already wounded, but after I successfully dodged the casters’ projectiles and slew them, Iona managed to evade hordes of enemy soldiers and make it back to my lines with about 1% health left. In one mission, I sent one of my shieldmaidens, Iona, on what I believed to be a suicide mission to hunt down some enemy casters. ![]() Even without any actual character development or personalities, they can start to take on a life of their own. This is further enhanced by the fact every unit is named, with a kill counter that persists across missions. With no reinforcements outside of certain rare scripted events, every unit is precious, and every death is felt. Instead, you’re simply given a small number of units and an objective. This style of games lacks the economic gameplay you see in most RTS titles. There’s really been nothing like them before or since. Nordic Warriors is obviously intended as a spiritual successor to Myth: The Fallen Lords and its sequel, a wildly unique series of real time strategy (or real time tactics, as they were sometimes dubbed) games from the 90s that I absolutely loved back in the day. After being intrigued by the demo, the developer of newly released RTS game Nordic Warriors was kind enough to give me a review code for the full version of the game. ![]() ![]() So the Dashware support stopped some time ago and currently the software seems bugged in a way you have to remove some DLLs, mess up with codecs and do some black magic in order to work, the downside of Dashware is that it only supports 1080p and the software crashes A LOT, there was one day that it was about 2 AM, I spent 2 hours doing the gauges and with an unsaved project the software crashed when I tried to render, reminding my 1990’s memories where hours of work would be thrown in the garbage due to software crashing. But GoPro guys (the company) bought Dashware and I think they hired the developers to do something for their Drone - the GoPro Karma. ![]() Not a problem: I partitioned my personal Mac with BootCamp, installed Windows 10 and installed the software. I joined Mavic Pilots and saw some telemetry posts and saw that guys use a Windows application called Dashware, which unfortunately works only for Windows. They created a software that gets metadata about their cameras’ files and embeds automatically in the video, and this is a thing that I always wanted to do because watching the video with these gauges and info allows me to discover what are the impacts of my flight controls in the final result (it’s like watching yourself presenting something), and work in these aspects to make a smoother footage. ![]() It took me approximately 18 working hours from zero knowledge about telemetry to this state: I was very very curious to know how to do that after seeing a Garmin (the GPS guys) video. ![]() ![]() ![]() “It is the first time that mythical ‘immortality medicines’ have been found in China,” said Shi Jiazhen, head of the Institute of Cultural Relics and Archaeology in Luoyang. The elixirs themselves, despite their widespread popularity among the dead, are not often found in their resting places. The last killed by the search for immortality was the Yongzheng Emperor in 1735. Qin Shi Huang, the first emperor of a united China who was entombed with the great Terracotta Army in a mausoleum complete with rivers of mercury, is said to have been the first emperor to die (in 210 B.C.) from elixir poisoning. Taiost literature records many nobles, even emperors, having died from elixir of life poisoning. Mercury and lead were the foundation of most formulae and it might seem ironic that they are both deadly poisons but it isn’t, really, because the power to end life was seen as the other side of their putative power to extend life. that describe the artificial creation of elixirs of life. There are surviving texts going back to the second century B.C. Waidan, meaning external elixir, was the earliest branch of Chinese alchemy, focusing on the achievement of immortality by combining metals and chemicals in a bronze crucible. These are two ingredients that according to ancient Taoist texts could be used to create an elixir of immortality. In fact it is a mixture of potassium nitrate and alunite, an aluminium potassium sulfate mineral. Laboratory testing on the fluid has found that the preliminary identification of grain wine was incorrect. The pot-bellied bronze vessel’s lid was still in place, and the seal was firm enough to prevent its evaporation. This find was of particular note because so much of the liquid had survived. Other tombs dating to the Western Han Dynasty (202 B.C.- 8 A.D.) have been found to include rice or sorghum wine stored in elaborate bronze vessels and the alcohol was known to have played an important ritual role in funerary practices of the period. It was yellow and smelled of alcohol, similar in appearance and scent to rice wine. When archaeologists discovered 3.5 liters of fluid in a bronze vessel in a 2,000-year-old tomb in Luoyang, central China, last October, they thought it was wine. Alchemists in various ages and cultures sought the means of formulating the elixir. This elixir was also said to cure all diseases. Another possibility is that the Fountain of Youth is a term limited only to the Funimation dub, as Funimation makes numerous alterations from the Japanese dub.The elixir of life, also known as elixir of immortality and sometimes equated with the philosopher’s stone, is a potion that supposedly grants the drinker eternal life and/or eternal youth. Otherwise, the Elixir may be an entirely different term than the Fountain. In several manga translations, it is stated that Master Roshi drank the "Immortality Elixir", which may indicate that the Elixir was formulated from ingredients and chemical compounds gathered from the Fountain of Youth. ![]() The Fountain of Youth originated from mythical tales in folklore, such as Greek and European accounts and theories.It is however revealed in the King Piccolo Saga that Roshi had lied about drinking the Elixir of Immortality, which is why he dies in battle with King Piccolo. It was first mentioned by Turtle, who argued against Master Roshi by stating that he had used the Fountain of Youth and therefore was not as old as he had informed Goku this was after Roshi had requested to see Bulma's breasts in exchange for the Bansho Fan and said it would be his last wish before he passes away. The elixir from the fountain made those who drank it completely Immortal - as it was said that if Roshi had drank it King Piccolo would be unable to kill him. ![]() The Fountain of Youth (also called the Elixir of Immortality) is a mythical fountain with mystical rejuvenating properties, which has the prophesied ability to restore a being's body to its prime, or at the very least, prevent them from fatalities such as death. ![]() ![]() Used in first-strand cDNA synthesis for subsequent NGS analysis. The reduced g-anisotropy relative to the CoCo enzyme and clearly axial symmetry of the CoCd EPR are consistent with the more centrosymmetric environment of the pseudotetrahedral Zn 1 site. Used in first-strand cDNA synthesis for downstream NGS applications.Īn optimised reverse transcriptase for production of full-length cDNA from as little as 100pg of total RNA/low abundance RNA. MMLV High Performance Reverse TranscriptaseĪn RNA-dependent MMLV DNA polymerase which is highly efficient at producing full-length cDNA from RNA templates. Used in the preparation of cDNA libraries for small-RNA transcriptome analysis (eg. Ligates single-stranded adenylated DNA or RNA oligos to RNA targets for a variety of applications. Useful for screening introns in cDNA libraries. Useful in removing rRNA from total RNA samples prior to library construction.Ī 3′ to 5′ exoribonuclease that digests linear RNAs but spares lariat or circular RNA. Useful in MIP-Seq and similar applications.ĭegrades RNA in a DNA:RNA hybrid without affecting DNA or any unhybridised RNA at high reaction temperatures. Highly thermostable DNA ligase for applications where ligation at high temperatures is beneficial. Used in combination with UNG (UDG) to generate a single nucleotide gap (with terminal 5' and 3' phosphates) at a uracil site. Used in combination with Endonuclease VIII to generate a single nucleotide gap (with terminal 5' and 3' phosphates) at a uracil site.Īcts as both an N-glycosylase and an AP-lyase in releasing damaged pyrimidines in DNA. The resulting abasic sites are susceptible to cleavage. ![]() Used in the circularisation of ssDNA that can be used as templates for rolling-circle replication.Īlso known as UDG, UNG catalyses the release of uracil from single and double stranded uracyl-containing DNA. Thermostable ligase that catalyses the intramolecular ligation of ssDNA templates. Used in second-strand cDNA synthesis and strand-displacement amplification. Adds an ʻAʼ base to the 3' end of the blunt phosphorylated DNA fragments, using the polymerase activity. Used in DNA sequencing to label the 5′ end of DNA and RNA with 32P or 33P.Įxo-Minus Klenow DNA Polymerase (D355A, E357A)Ī Klenow DNA polymerase that lacks all 5′ to 3′ and 3′ to 5′ exonuclease activity. Phosphorylates 5′ ends of ssDNA, dsDNA, RNA and nucleoside 3′ monophosphates. ![]() Used to form blunt ends by removing 3′ overhangs or filling in 5′ overhangs. Workhorse enzyme for adapter ligation to build complex libraries.Īn E.coli DNA polymerase I fragment that lacks 5′ to 3′ exonuclease activity but retains 3′ to 5′ exonuclease activity and 5′ to 3′ polymerase activity. T4 DNA Ligase catalyses the formation of a phosphodiester bond between the terminal 5′ phosphate and 3′ hydroxyl groups of duplex DNA or RNA. ![]() Start with a small batch for your pilot studies and scale up to bulk orders when you’re ready. ![]() |
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